This study was performed for the purpose of detecting the effect of artificially induced bile duct injury to the survival, growth and development of Clonorchis sinensis.
Escherichia coli suspension was administered to common bile duct of rat infected with C. sinensis. Then, the worm recovery rate, the changes in mean number of eggs per gram of feces, the size and the morphological changes of the recovered worms were observed.
Experimental groups were divided as follows;
Group 1: E. coli administration was executed at 4 weeks after about 60 metacercariae feeding.
Group 2: E. coli administration was executed at 1 week after about 60 metacercariae feeding.
Group 3(Control): About 60 metacercariae of C. sinensis were infected without E. coli administration.
The following results were acquired from this experiment.;
1. The worm recovery rates of Group 1 (6.6,x) and Group 2 (14.6%) were lower than that of Group 3 (87.4%).
2. There was no difference in size of the worms of Group 1 and control. But marked
retardation of growth was observed in the recovered worms from Group 2.
3. Disintegration of testis, ovary and uterus was observed in the recovered worms of
Group 1. But in Group 2, development of organ was nearly normal although the size
was unusually small.
Eggs were not detected in stools of Group 1 since 2nd week of E. coli administration. Small worms recovered from Group 2 were observed numerous eggs in their uterus, however in the stool examination with the application of concentration method the eggs were not detected until 12th week of C. sinensis infection.
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